Chem-0199 is an inhibitor of adipocyte enhancer-binding protein 1 (AEBP1). It disrupts the interaction between AEBP1 and CKAP4, which enhances antitumor immunity. Chem-0199 can inhibit Akt phosphorylation (p-Akt) and downregulate PD-L1 expression. It is applicable in cancer research, including studies on colorectal cancer.

Chem-0199 inhibits the binding of AEBP1 and CKAP4 in human and mouse cancer-associated fibroblasts (hCAFs/mCAFs). At concentrations of 10-100 μM, Chem-0199 suppresses Akt phosphorylation (p-Akt) and reduces PD-L1 expression in mCAFs. Furthermore, at doses of 10-100 μM over 48 hours, it significantly increases the ratio of IFN-γ+ and TNF-α+ CD8+ T cells in mouse spleens, restoring T cell cytotoxicity. Chem-0199 at 1-20 μM for 72 hours does not significantly inhibit the proliferation of various tumor cells (MC38, CT26, B16-F10).

Chem-0199, when administered via intraperitoneal injection at doses ranging from 3 to 30 mg/kg, is capable of inhibiting the growth of MC38 tumors in wild-type C57BL/6 mice.