C12-iE-DAP hydrochloride is the hydrochloride salt form of C12-iE-DAP and is a biologically active peptide that functions as an agonist of NOD1, making it a useful research reagent for investigating innate immune recognition, pattern recognition receptor signaling, and host–pathogen interaction pathways.

NOD1:0.027 nM

Method: HEK293 cells were engineered using a lentiviral NF-κB–dependent β-galactosidase reporter system to measure NOD1 receptor activation.
Result: NOD1 ligand stimulation activated NF-κB signaling in HEK293 cells, enabling quantitative assessment of receptor–ligand interactions via β-galactosidase activity[1].

Method: C12-iE-DAP hydrochloride (200 μg/mouse) or TLR4 ligand (5 μg/mouse) was administered intramuscularly in mice, and NF-κB activation was measured across organs.
Result: C12-iE-DAP hydrochloride induced maximal NF-κB activation in the liver and small intestine at 5 h post-injection, with minimal activation in kidneys, heart, and small intestine relative to other organs.

DMSO: 80.00 mg/mL, Sonication is recommended.