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Brilliant Blue FCF

Name: Brilliant Blue FCF
Brand: TargetMol
SKU: T7771
Rating: 4.5 (1 reviews)

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Catalog No. T7771Cas No. 3844-45-9

Alias FD&C Blue No. 1, Erioglaucine disodium salt, E133, Acid Blue 9

Brilliant Blue FCF (Erioglaucine disodium salt) is a nontoxic dye for saphenous vein graft marking that abrogates response to injury.

Brilliant Blue FCF

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Purity: 99.38%

Catalog No. T7771Alias FD&C Blue No. 1, Erioglaucine disodium salt, E133, Acid Blue 9Cas No. 3844-45-9

Brilliant Blue FCF (Erioglaucine disodium salt) is a nontoxic dye for saphenous vein graft marking that abrogates response to injury.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
10 g	$42	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.38%

Appearance:Solid

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COA LCMS HNMR

Product Introduction

Brilliant Blue FCF AI Summary

Brilliant Blue FCF inhibits mouse Panx-1 channels expressed in Xenopus laevis oocytes. This inhibition was evaluated using a Whole cell voltage clamp assay by measuring the reduction of membrane current in cells maintained at -60 mV and exposed to voltage steps to +60 mV in the presence of CBX. The compound exhibited an IC50 value of 270.0 nM for this activity..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Brilliant Blue FCF (Erioglaucine disodium salt) is a nontoxic dye for saphenous vein graft marking that abrogates response to injury.
Cell Research	Instructions 1. Solvent selection: Brilliant Blue FCF is soluble in water, usually in deionized or distilled water. The common concentration range is 0.01% to 0.1% (w/v), and the specific concentration depends on the application requirements. 2. Use in experiments: a. Experimental staining: In biological research, Brilliant Blue FCF can be used for laboratory staining, such as as a tracer in electrophoresis experiments to help monitor the migration or separation process of samples. b. pH indicator: In the pH range of 4 to 5, Brilliant Blue FCF shows blue and can be used as an indicator for pH indication of buffers. c. Staining experiments: As a dye, Brilliant Blue FCF can be used to stain cell or tissue samples. The staining method is usually to mix the dye solution with the sample to be stained, incubate for a certain period of time at room temperature, and then detect the staining results by microscope or spectrophotometer. Note: 1) Brilliant Blue FCF should be stored in a dry and cool place to avoid contact with strong acids or bases. 2) For laboratory operations, appropriate protective equipment such as gloves and goggles should be worn. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	FD&C Blue No. 1, Erioglaucine disodium salt, E133, Acid Blue 9

Chemical Properties

Molecular Weight	792.85
Formula	C₃₇H₃₄N₂Na₂O₉S₃
Cas No.	3844-45-9
Smiles	[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c1ccc(cc1)C(=C1C=CC(C=C1)=[N+](CC)Cc1cccc(c1)S([O-])(=O)=O)c1ccccc1S([O-])(=O)=O
Relative Density.	1 480 kg/m3. Temperature:23 °C.

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 33.33 mg/mL (42.04 mM), when pH is adjusted to 11 with NaOH. Sonication is recommended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	1.2613 mL	6.3064 mL	12.6127 mL	63.0636 mL
5 mM	0.2523 mL	1.2613 mL	2.5225 mL	12.6127 mL
10 mM	0.1261 mL	0.6306 mL	1.2613 mL	6.3064 mL
20 mM	0.0631 mL	0.3153 mL	0.6306 mL	3.1532 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc