Brilliant Blue FCF (Erioglaucine disodium salt) is a nontoxic dye for saphenous vein graft marking that abrogates response to injury.

Instructions
1. Solvent selection:
Brilliant Blue FCF is soluble in water, usually in deionized or distilled water. The common concentration range is 0.01% to 0.1% (w/v), and the specific concentration depends on the application requirements.
2. Use in experiments:
a. Experimental staining: In biological research, Brilliant Blue FCF can be used for laboratory staining, such as as a tracer in electrophoresis experiments to help monitor the migration or separation process of samples.
b. pH indicator: In the pH range of 4 to 5, Brilliant Blue FCF shows blue and can be used as an indicator for pH indication of buffers.
c. Staining experiments:
As a dye, Brilliant Blue FCF can be used to stain cell or tissue samples. The staining method is usually to mix the dye solution with the sample to be stained, incubate for a certain period of time at room temperature, and then detect the staining results by microscope or spectrophotometer.
Note:
1) Brilliant Blue FCF should be stored in a dry and cool place to avoid contact with strong acids or bases.
2) For laboratory operations, appropriate protective equipment such as gloves and goggles should be worn.