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BRD5529

Name: BRD5529
Brand: TargetMol
SKU: T14777
Price: 52 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: BRD-5529, BRD 5529) Copy Product Info

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Synonyms: BRD-5529, BRD 5529

Catalog No. T14777 Copy Product Info

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BRD5529 is a selective inhibitor of the CARD9-E3 ubiquitin ligase TRIM62 protein-protein interaction, with an IC50 value of 8.6 μM. BRD5529 directly and selectively binds CARD9, disrupts TRIM62 recruitment, inhibits TRIM62-mediated ubiquitinylation, and suppresses CARD9 activation. BRD5529 is therefore applicable to investigations involving innate immune signaling, ubiquitination pathways, inflammatory regulation, and CARD9-associated immune responses.

Cas No. 1358488-78-4

Pack Size	Price	USA Stock	Global Stock	Quantity
2 mg	$52	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Appearance:Solid

Color:Gray

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Product Introduction

Bioactivity

Description	BRD5529 is a selective inhibitor of the CARD9-E3 ubiquitin ligase TRIM62 protein-protein interaction, with an IC50 value of 8.6 μM. BRD5529 directly and selectively binds CARD9, disrupts TRIM62 recruitment, inhibits TRIM62-mediated ubiquitinylation, and suppresses CARD9 activation. BRD5529 is therefore applicable to investigations involving innate immune signaling, ubiquitination pathways, inflammatory regulation, and CARD9-associated immune responses.
Targets & IC50	CARD9-TRIM62:8.6 μM
In vitro	BRD5529 exhibits potent dose-dependent inhibitory activity against the CARD9-TRIM62 interaction, with an IC50 value of 8.6 μM[1]. BRD5529 (200 μM, 0–50 min; 200 μM, 2–4 h) suppresses CARD9-dependent signaling in innate immune cells[1]. BRD5529 (40 μM) dose-dependently inhibits TRIM62-mediated CARD9 ubiquitination in vitro[1].
In vivo	BRD5529 (0.1 or 1.0 mg/kg; i.p.; daily, for 2 weeks) does not produce significant changes in daily or final weight gain, shows no major differences in proinflammatory cytokine levels, and does not cause significant alterations in lung, liver, or kidney pathology scores of Pneumocystis pneumonia (PCP) mice model[2].
Synonyms	BRD-5529, BRD 5529

Chemical Properties

Molecular Weight	465.55
Formula	C₂₅H₃₁N₅O₄
Cas No.	1358488-78-4
Smiles	O=C(O)C1=CC(=CN=C1N2CCC(C(=O)N)(N3CCCCC3)CC2)NC(=O)C4=CC=C(C=C4)C
Relative Density.	1.327 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (214.8 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1480 mL	10.7400 mL	21.4800 mL	107.3998 mL
5 mM	0.4296 mL	2.1480 mL	4.2960 mL	21.4800 mL
10 mM	0.2148 mL	1.0740 mL	2.1480 mL	10.7400 mL
20 mM	0.1074 mL	0.5370 mL	1.0740 mL	5.3700 mL
50 mM	0.0430 mL	0.2148 mL	0.4296 mL	2.1480 mL
100 mM	0.0215 mL	0.1074 mL	0.2148 mL	1.0740 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc