• Modeling Mechanism：

  Bovine Type II Collagen, as a foreign antigen, induced mice to produce anti-type II collagen-specific IgG2a antibodies after emulsification with complete Freund's adjuvant (FCA), activating an autoimmune response. By activating the NF-κB and MAPK (ERK1/2, p38) signaling pathways, it promoted the release of pro-inflammatory factors TNF-α and IL-6, leading to neutrophil infiltration, cell proliferation, and joint damage in joint tissues.

• Related Products：

  Bovine Type II Collagen (T80025)

• Modeling Method：

  Experimental Subject：

  Mice, DBA/1, Male, 8 weeks old

  Dosage and Administration Route：

  ① Primary immunisation: Bovine Type II Collagen, emulsified in complete Freund's adjuvant (FCA), subcutaneous injection (Day 0);
  ② Booster immunisation: Same dose Bovine Type II Collagen+FCA, Subcutaneous injection (Day 21);
  ③ Intervention control (optional): 10 mg/kg high-density lipoprotein (HDL), Intravenous injection via tail vein (from Day 28)

  Dosing Frequency and Duration Model：

  Primary and booster immunisations: single dose; Intervention control: once every 3 days for 5 doses total

• Validation：

  Clinical phenotype: Significant joint swelling (increased claw thickness) appeared after day 28, with elevated arthritis scores (up to 10 points) and weight loss; Molecular markers: Serum TNF-α and IL-6 levels were significantly elevated (TNF-α: 112.6±9.373 pg/mL, IL-6: 72.74±8.803 pg/mL), and anti-type II collagen IgG2a antibody levels were upregulated; Histological markers: HE staining of joint tissue showed neutrophil infiltration, cell proliferation, disordered arrangement, and joint structural destruction; Signaling pathways: Significantly elevated expression of NF-κB p65, p-IκBα, and MAPK pathway p-ERK1/2 and p-p38 proteins in joint tissue.

*Precautions： They were put to death 60 days after the model was created.

*References：Wang Y,et,al. Anti-Inflammatory Effects of HDL in Mice With Rheumatoid Arthritis Induced by Collagen. Front Immunol. 2018 May 11;9:1013.