BODIPY TR Cadaverine

BODIPY TR Cadaverine, a derivative of cadaverine, is a red fluorescent dye used in a highly sensitive and robust fluorescent displacement assay. BODIPY TR Cadaverine binds strongly to native LPS, specifically recognizing lipid A, and is competitively displaced by compounds that have an affinity for lipid A. BODIPY TR Cadaverine is therefore used in bacterial membrane biology and endotoxin-binding assay systems to study lipid A interactions, LPS binding competition, and small-molecule displacement dynamics in fluorescence-based biochemical screening platforms.

BODIPY TR Cadaverine assay [1] involves quantifying autophagy in cells by incubating them with 125 nM of the compound for 10 minutes. After incubation, the cells are washed four times with PBS and lysed using a buffer containing 10 mM Tris-Cl pH 8 and 0.1% Triton X-100. Fluorescence measurements are then taken with a SPECTRAmax fluorimeter (Molecular Devices) at excitation and emission wavelengths of 588 nm and 616 nm, respectively. To normalize fluorescence levels relative to cell number, 0.2 mM ethidium bromide is added, and DNA fluorescence is read at an excitation wavelength of 530 nm and emission wavelength of 590 nm [1].

DMSO: 100 mg/mL (183.54 mM), Sonication is recommended.