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BODIPY-Cholesterol
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Catalog No. T41008Cas No. 878557-19-8
Alias BCh2
BODIPY-Cholesterol (TF-Chol) is a cholesterol analog with a fluorescent BODIPY motif and cellular permeability, and is a novel BODIPY cholesterol probe.BODIPY-Cholesterol can be used to study intracellular sterol uptake and interorganelle sterol flux.
BODIPY-Cholesterol
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Purity: 98.79%
Catalog No. T41008Alias BCh2Cas No. 878557-19-8
BODIPY-Cholesterol (TF-Chol) is a cholesterol analog with a fluorescent BODIPY motif and cellular permeability, and is a novel BODIPY cholesterol probe.BODIPY-Cholesterol can be used to study intracellular sterol uptake and interorganelle sterol flux.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 1 mg | $179 | - | In Stock | |
| 5 mg | $347 | - | In Stock | |
| 10 mg | $625 | - | In Stock | |
| 25 mg | $972 | - | In Stock | |
| 50 mg | $1,280 | - | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Batch Information
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Purity:98.79%
Appearance:Solid
Color:Orange
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | BODIPY-Cholesterol (TF-Chol) is a cholesterol analog with a fluorescent BODIPY motif and cellular permeability, and is a novel BODIPY cholesterol probe.BODIPY-Cholesterol can be used to study intracellular sterol uptake and interorganelle sterol flux. |
| In vitro | For the BODIPY-cholesterol assay:Reconstitute the analogue in DMSO to prepare a stock solution of BODIPY-cholesterol at a concentration of 1-10 μM[2]. Dilute cells with PBS (or other buffers), wash them (300×g, 10 min) to eliminate excess diluent, and resuspend the loose pellet with vehicle media to achieve the desired concentration[2].Label cell suspensions with BODIPY-cholesterol (final concentration of 0.5-5 μM), ensuring thorough mixing, and incubate for 10-20 minutes at 37 °C[1].Dilute the cells with each of the capacitation media conditions, as outlined in Incubation media, to a final concentration. Incubate for 2 hours before flow cytometric assessment[2]. |
| Synonyms | BCh2 |
| Molecular Weight | 576.61 |
| Formula | C36H51BF2N2O |
| Cas No. | 878557-19-8 |
| Smiles | [H][C@@]1(CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C)[C@H](C)CCC1=C2C(C)=CC(C)=[N+]2[B-](F)(F)n2c(C)cc(C)c12 |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | ||||||||||
| Solubility Information | DMSO: 1 mg/mL (1.73 mM), Sonication is recommended. H2O: <0.1 mg/mL (Insoluble) | ||||||||||
Solution Preparation Table | |||||||||||
DMSO
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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