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BODIPY 493/503 NHS ester

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Catalog No. T205829Cas No. 216961-98-7

BODIPY 493/503 NHS ester is a widely used reagent for conjugating this dye to proteins or antibodies. The NHS ester reacts with primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting BODIPY 493/503 conjugates exhibit bright fluorescence, narrow emission bandwidth, and relatively long excited-state lifetimes, which are suitable for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Ex/Em(nm) = 496/508.

BODIPY 493/503 NHS ester

BODIPY 493/503 NHS ester

😃Good
Catalog No. T205829Cas No. 216961-98-7
BODIPY 493/503 NHS ester is a widely used reagent for conjugating this dye to proteins or antibodies. The NHS ester reacts with primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting BODIPY 493/503 conjugates exhibit bright fluorescence, narrow emission bandwidth, and relatively long excited-state lifetimes, which are suitable for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Ex/Em(nm) = 496/508.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
1 mg$1957-10 days7-10 days
5 mg$4837-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
BODIPY 493/503 NHS ester is a widely used reagent for conjugating this dye to proteins or antibodies. The NHS ester reacts with primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting BODIPY 493/503 conjugates exhibit bright fluorescence, narrow emission bandwidth, and relatively long excited-state lifetimes, which are suitable for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Ex/Em(nm) = 496/508.
Chemical Properties
Molecular Weight417.21
FormulaC20H22BF2N3O4
Cas No.216961-98-7
SmilesO=C(CCC1=C2C(C)=CC(C)=[N+]2[B-](F)(N3C1=C(C)C=C3C)F)ON4C(CCC4=O)=O
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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