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BODIPY 493/503 NHS ester is a widely used reagent for conjugating this dye to proteins or antibodies. The NHS ester reacts with primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting BODIPY 493/503 conjugates exhibit bright fluorescence, narrow emission bandwidth, and relatively long excited-state lifetimes, which are suitable for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Ex/Em(nm) = 496/508.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 1 mg | $195 | 7-10 days | 7-10 days | |
| 5 mg | $483 | 7-10 days | 7-10 days |
| Description | BODIPY 493/503 NHS ester is a widely used reagent for conjugating this dye to proteins or antibodies. The NHS ester reacts with primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting BODIPY 493/503 conjugates exhibit bright fluorescence, narrow emission bandwidth, and relatively long excited-state lifetimes, which are suitable for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Ex/Em(nm) = 496/508. |
| Molecular Weight | 417.21 |
| Formula | C20H22BF2N3O4 |
| Cas No. | 216961-98-7 |
| Smiles | O=C(CCC1=C2C(C)=CC(C)=[N+]2[B-](F)(N3C1=C(C)C=C3C)F)ON4C(CCC4=O)=O |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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