ATPase-IN-8

ATPase-IN-8, an anti-enterovirus agent, functions by inhibiting the viral non-structural protein 2C and has an IC50 of 1.4 μM against CV-B3 2C ATPase. It exhibits significant antiviral activity specifically against enteroviruses, acting as a potent and selective inhibitor of CV-B3 and EV-D68. This compound is useful for researching enterovirus infections.

ATPase-IN-8 (Compound 53) effectively inhibits the replication of CV-B3 in HeLa CCL-2 cells with an EC50 of 0.5 μM, CC50 of 22 μM, and a selectivity index of 44 when used for 72 hours. In RD cells, ATPase-IN-8 inhibits EV-D68 replication with an EC50 of 0.4 μM, CC50 of 23 μM, and a selectivity index of 58 over a period of 72 hours. It also suppresses EV-A71 replication in RD cells with an EC50 of 8 μM, CC50 of 23 μM, and a selectivity index of 3 within 48-72 hours. ATPase-IN-8 further prevents the cytopathic effect induced by EV-D68 in RD cells, showing an EC50 of 0.6 μM at 72 hours. Additionally, ATPase-IN-8 (0.1-10 μM; 3 days) reduces plaque formation of EV-D68 in a dose-dependent manner in RD cells, achieving complete inhibition at concentrations of 1 μM and above.