Apoptosis inducer45 is an apoptosis inducer demonstrating cytotoxic activity against the MCF-7 cell line. It initiates apoptosis in these cells via the mitochondrial pathway by increasing the Bax/Bcl-2 ratio, leading to the cleavage of caspase-9 and subsequent fragmentation of the DNA repair protein PARP. Additionally, Apoptosis inducer45 induces the cleavage of caspase-8, which subsequently triggers the cleavage of caspase-3 and its downstream target PARP, resulting in extrinsic apoptosis. This compound is applicable in breast cancer research.

Apoptosis inducer 45 (Compound 11s) inhibits cell proliferation with IC 50 values of 3.56, 4.93, 7.76, and 13.35 μM for A549, MCF-7, HL-7702, and MDA-MB-231 cells, respectively. At concentrations of 2.5-10 μM over 24 hours, it induces apoptosis in MCF-7 cells through both extrinsic and intrinsic pathways. Additionally, Apoptosis inducer 45 exhibits concentration- and time-dependent inhibitory effects on MCF-7 cell growth and colony formation over 2.5-10 μM and 24 hours to 14 days. At 2.5-10 μM for 72 hours, it significantly reduces the migratory ability of MCF-7 cells.