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Anti-Phospho-BTK (Tyr223) Antibody (3S713)

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Catalog No. TMAB-01397

Anti-Phospho-BTK (Tyr223) Antibody (3S713) is a Rabbit antibody targeting Phospho-BTK (Tyr223). Anti-Phospho-BTK (Tyr223) Antibody (3S713) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-Phospho-BTK (Tyr223) Antibody (3S713)

Anti-Phospho-BTK (Tyr223) Antibody (3S713)

😃Good
Catalog No. TMAB-01397
Anti-Phospho-BTK (Tyr223) Antibody (3S713) is a Rabbit antibody targeting Phospho-BTK (Tyr223). Anti-Phospho-BTK (Tyr223) Antibody (3S713) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$271 7-10 days
100 μL$489 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: WB,IHC-P,IHC-Fr,ICC/IF,IF

Reactivity: Human (predicted:Mouse,Rat)
Ig Type
IgG
Clone
3S713
Reactivity
Human (predicted:Mouse,Rat)
Verified Activity
Western blot analysis of Phospho-BTk (Y223) on different lysates using anti-Phospho-BTk (Y223) antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562 cells treated with pervanadate
Lane 2: Untreated K562 cell lysate
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:1000-2000; IHC-P: 1:100-500; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Note=In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human Btk around the phosphorylation site of Tyr223
Antigen Species
Human
Gene ID
695
Uniprot ID
Biology Area
TLR Signaling,Tyrosine Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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