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Anti-Phospho-BTK (Tyr223) Antibody (3S713) is a Rabbit antibody targeting Phospho-BTK (Tyr223). Anti-Phospho-BTK (Tyr223) Antibody (3S713) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $271 | 7-10 days | |
100 μL | $489 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,ICC/IF,IF Reactivity: Human (predicted:Mouse,Rat) |
Ig Type | IgG |
Clone | 3S713 |
Reactivity | Human (predicted:Mouse,Rat) |
Verified Activity | Western blot analysis of Phospho-BTk (Y223) on different lysates using anti-Phospho-BTk (Y223) antibody at 1/1,000 dilution. Positive control: Lane 1: K562 cells treated with pervanadate Lane 2: Untreated K562 cell lysate |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
Recommended Dose | WB: 1:1000-2000; IHC-P: 1:100-500; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Note=In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1. |
Construction | Recombinant Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated Synthesised phosphopeptide: human Btk around the phosphorylation site of Tyr223 |
Antigen Species | Human |
Gene ID | 695 |
Uniprot ID | |
Biology Area | TLR Signaling,Tyrosine Kinases |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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