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Anti-MMP-13 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-01152
Anti-MMP-13 Polyclonal Antibody 2 is a Rabbit antibody targeting MMP-13. Anti-MMP-13 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-MMP-13 Polyclonal Antibody 2
Anti-MMP-13 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-01152
Anti-MMP-13 Polyclonal Antibody 2 is a Rabbit antibody targeting MMP-13. Anti-MMP-13 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Rabbit) |
| Verified Activity | 1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-MMP13 Polyclonal Antibody, Unconjugated (TMAB-01152) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-MMP13 Polyclonal Antibody, Unconjugated (TMAB-01152) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Mg63 (Human) Cell Lysate at 30 μg Primary: Anti-MMP13 (TMAB-01152) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 63 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix (Probable). |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human MMP13 |
| Antigen Species | Human |
| Gene ID | 4322 |
| Uniprot ID | |
| Biology Area | MMPs,MMPs,MMPs,MMP,MMPs,Tumor biomarkers ELISA kits,MMP |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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