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Anti-CES1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00418

Anti-CES1 Polyclonal Antibody is a Rabbit antibody targeting CES1. Anti-CES1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CES1 Polyclonal Antibody

Anti-CES1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00418
Anti-CES1 Polyclonal Antibody is a Rabbit antibody targeting CES1. Anti-CES1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CES1 Polyclonal Antibody is a Rabbit antibody targeting CES1. Anti-CES1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Pig,Cow,Rabbit)
Verified Activity
1. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CES1 Polyclonal Antibody, Unconjugated (TMAB-00418) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CES1/ Liver Carboxylesterase 1 Polyclonal Antibody, Unconjugated (TMAB-00418) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Protein: intestinal (mouse) lysate at 40 μg;
Primary: rabbit Anti-CES1/Liver Carboxylesterase 1 (TMAB-00418) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000;
Predicted band size: 61 kDa
Observed band size: 66 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum lumen.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CES1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Lipoprotein metabolism,Cholesterol Metabolism,Hydrolysis,Lipoproteins/Apolipoproteins,Drug metabolism,Cholesterol Metabolism,Hydrolysis,Lipoprotein metabolism,Cancer,Heart disease,Drug metabolism,Lipid metabolism
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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