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HEPES Sodium

Catalog No. T22839 CAS 75277-39-3

HEPES Sodium salt is a nonvolatile zwitterionic chemical buffering agent. HEPES Sodium salt is broadly applied in cell culture. HEPES Sodium salt is effective at pH 6.8 to 8.2. HEPES Sodium salt also induces of lysosome biogenesis.

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HEPES Sodium, CAS 75277-39-3

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10 g	In stock	$ 36.00

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Purity: 99.9%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

In vitro

HEPES Sodium salt maintains superhydrophilicity of titanium for at least 3 months, leading to a continuous retention of bioactivity and osteoconductivity[1]. In cultured RAW264.7 cells, HEPES Sodium salt drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, disrupts global cellular transcriptional profiles, leading to the activation of a MiT/TFE-dependent lysosomal-autophagic gene network[3].

Molecular Weight	260.29
Formula	C8H17N2NaO4S
CAS No.	75277-39-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: Soluble

DMSO: 50 mg/mL (192.09 mM), Sonication is recommended.

References and Literature

1. Suzuki T, et al. Nonvolatile buffer coating of titanium to prevent its biological aging and for drug delivery. Biomaterials. 2010;31(18):4818-4828. 2. Sledź P, et al. An experimental charge density of HEPES. Acta Crystallogr B. 2010;66(Pt 4):482-492. 3. Tol MJ, van der Lienden MJC, Gabriel TL, Hagen JJ, Scheij S, Veenendaal T, Klumperman J, Donker-Koopman WE, Verhoeven AJ, Overkleeft H, Aerts JM, Argmann CA, van Eijk M. HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution. Autophagy. 2018;14(3):437-449. doi: 10.1080/15548627.2017.1419118. Epub 2018 Feb 17. PMID: 29455584; PMCID: PMC5915011.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

HEPES Sodium 75277-39-3 Others HEPES Sodium salt inhibitor inhibit

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