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5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

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Catalog No. T85479Cas No. 15548-60-4
Alias X-glucoside, X-Glc

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside (X-Glc) is a chromogenic substrate for β-galactosidase, producing a blue product under β-galactosidase catalysis, commonly used for in situ β-galactosidase staining and blue-white screening.

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

😃Good
Catalog No. T85479Alias X-glucoside, X-GlcCas No. 15548-60-4
5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside (X-Glc) is a chromogenic substrate for β-galactosidase, producing a blue product under β-galactosidase catalysis, commonly used for in situ β-galactosidase staining and blue-white screening.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
200 mg$31In StockIn Stock
500 mg$53In StockIn Stock
1 g$74In StockIn Stock
2 g$108-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside (X-Glc) is a chromogenic substrate for β-galactosidase, producing a blue product under β-galactosidase catalysis, commonly used for in situ β-galactosidase staining and blue-white screening.
In vitro
5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside is a biochemical reagent used as a biomaterial or organic compound in life sciences research.
SynonymsX-glucoside, X-Glc
Chemical Properties
Molecular Weight408.63
FormulaC14H15BrClNO6
Cas No.15548-60-4
SmilesO(C=1C=2C(NC1)=CC=C(Br)C2Cl)[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O
ColorWhite
AppearanceSolid
Storage & Solubility Information
Storagestore at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 80 mg/mL (195.78 mM), Sonication is recommended.
In Vivo Formulation
10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (8.08 mM), Sonication is recommeded.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.4472 mL12.2360 mL24.4720 mL122.3601 mL
5 mM0.4894 mL2.4472 mL4.8944 mL24.4720 mL
10 mM0.2447 mL1.2236 mL2.4472 mL12.2360 mL
20 mM0.1224 mL0.6118 mL1.2236 mL6.1180 mL
50 mM0.0489 mL0.2447 mL0.4894 mL2.4472 mL
100 mM0.0245 mL0.1224 mL0.2447 mL1.2236 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.
1 Enter information below:
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2 Enter the in vivo formulation:
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%
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