5-Aminofluorescein (5-AF) (5-AF) is a covalently-bound fluorescence marker conjugated with human serum albumin. It exhibits an excitation wavelength of 490 nm and an emission wavelength of 515 nm.

Instructions for use
I. Preparation of mother solution and working solution: Dissolve 5-Aminofluorescein5 mother solution with DMSO to prepare a suitable concentration, and dilute it with a suitable solvent to a certain concentration of working solution when using; (be careful to store in a dark place)
II. Labeling reaction
1. Chemically couple 5-Aminofluorescein with target molecules (such as proteins, nucleic acids or other biomolecules).
2. Detection: Use fluorescence microscope or fluorescence spectrophotometer for detection. The excitation wavelength of 5-Aminofluorescein5 is usually about 490 nm, and the emission wavelength is about 515 nm.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

DMSO: 30 mg/mL (86.38 mM), Sonication is recommended.