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5-Aminofluorescein (5-AF) (5-AF) is a covalently-bound fluorescence marker conjugated with human serum albumin. It exhibits an excitation wavelength of 490 nm and an emission wavelength of 515 nm.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
200 mg | $32 | In Stock | |
1 mL x 10 mM (in DMSO) | $50 | In Stock |
Description | 5-Aminofluorescein (5-AF) (5-AF) is a covalently-bound fluorescence marker conjugated with human serum albumin. It exhibits an excitation wavelength of 490 nm and an emission wavelength of 515 nm. |
In vitro | Instructions for use I. Preparation of mother solution and working solution: Dissolve 5-Aminofluorescein5 mother solution with DMSO to prepare a suitable concentration, and dilute it with a suitable solvent to a certain concentration of working solution when using; (be careful to store in a dark place) II. Labeling reaction 1. Chemically couple 5-Aminofluorescein with target molecules (such as proteins, nucleic acids or other biomolecules). 2. Detection: Use fluorescence microscope or fluorescence spectrophotometer for detection. The excitation wavelength of 5-Aminofluorescein5 is usually about 490 nm, and the emission wavelength is about 515 nm. |
Alias | 5-AF |
Molecular Weight | 347.32 |
Formula | C20H13NO5 |
Cas No. | 3326-34-9 |
Smiles | NC1=CC2=C(C=C1)C1(OC2=O)C2=C(OC3=C1C=CC(O)=C3)C=C(O)C=C2 |
Relative Density. | 1.64g/cm3 |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||||||||||||||||||||||
Solubility Information | DMSO: 30 mg/mL (86.38 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||
DMSO
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