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5-Aminofluorescein

Name: 5-Aminofluorescein
Brand: TargetMol
SKU: T18889
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T18889Cas No. 3326-34-9

Alias 5-AF

5-Aminofluorescein (5-AF) (5-AF) is a covalently-bound fluorescence marker conjugated with human serum albumin. It exhibits an excitation wavelength of 490 nm and an emission wavelength of 515 nm.

5-Aminofluorescein

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Purity: 98.59%

Catalog No. T18889Alias 5-AFCas No. 3326-34-9

5-Aminofluorescein (5-AF) (5-AF) is a covalently-bound fluorescence marker conjugated with human serum albumin. It exhibits an excitation wavelength of 490 nm and an emission wavelength of 515 nm.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
200 mg	$29	In Stock	-
1 mL x 10 mM (in DMSO)	$30	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:98.59%

Appearance:Solid

Color:Black

Resource Download

COA HPLC HNMR

Product Introduction

5-Aminofluorescein AI Summary

5-Aminofluorescein inhibits the N-terminal 31 residues truncated FTO demethylase activity using m6A-containing ssRNA as a substrate, with an IC50 value of 6550.0 nM, as determined by an assay using LC-MS analysis over a 1-hour incubation period..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	5-Aminofluorescein (5-AF) (5-AF) is a covalently-bound fluorescence marker conjugated with human serum albumin. It exhibits an excitation wavelength of 490 nm and an emission wavelength of 515 nm.
In vitro	Instructions for use I. Preparation of mother solution and working solution: Dissolve 5-Aminofluorescein5 mother solution with DMSO to prepare a suitable concentration, and dilute it with a suitable solvent to a certain concentration of working solution when using; (be careful to store in a dark place) II. Labeling reaction 1. Chemically couple 5-Aminofluorescein with target molecules (such as proteins, nucleic acids or other biomolecules). 2. Detection: Use fluorescence microscope or fluorescence spectrophotometer for detection. The excitation wavelength of 5-Aminofluorescein5 is usually about 490 nm, and the emission wavelength is about 515 nm. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	5-AF

Chemical Properties

Molecular Weight	347.32
Formula	C₂₀H₁₃NO₅
Cas No.	3326-34-9
Smiles	NC1=CC2=C(C=C1)C1(OC2=O)C2=C(OC3=C1C=CC(O)=C3)C=C(O)C=C2
Relative Density.	1.64g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 30 mg/mL (86.38 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8792 mL	14.3959 mL	28.7919 mL	143.9595 mL
5 mM	0.5758 mL	2.8792 mL	5.7584 mL	28.7919 mL
10 mM	0.2879 mL	1.4396 mL	2.8792 mL	14.3959 mL
20 mM	0.1440 mL	0.7198 mL	1.4396 mL	7.1980 mL
50 mM	0.0576 mL	0.2879 mL	0.5758 mL	2.8792 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc