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2'-O-Methyl-5-methylcytidine

Name: 2'-O-Methyl-5-methylcytidine
Brand: TargetMol
SKU: TNU0124
Price: 35 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. TNU0124Cas No. 113886-70-7

Alias 5-Methyl-2′-O-methylcytidine

2'-O-Methyl-5-methylcytidine is a naturally occurring modified nucleoside found in tRNA that features methylation at both the ribose 2'-hydroxyl position and the cytosine 5-position, conferring enhanced stability and potential antiviral and anticancer properties, 2'-O-Methyl-5-methylcytidine is widely used as a phosphoramidite building block for synthesizing modified RNA in drug discovery and structural biology research.

2'-O-Methyl-5-methylcytidine

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Purity: 99.88%

Catalog No. TNU0124Alias 5-Methyl-2′-O-methylcytidineCas No. 113886-70-7

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$35	-	In Stock
5 mg	$53	-	In Stock
10 mg	$75	-	In Stock
25 mg	$122	-	In Stock
50 mg	$178	-	In Stock
100 mg	$262	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.88%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	2'-O-Methyl-5-methylcytidine is a naturally occurring modified nucleoside found in tRNA that features methylation at both the ribose 2'-hydroxyl position and the cytosine 5-position, conferring enhanced stability and potential antiviral and anticancer properties, 2'-O-Methyl-5-methylcytidine is widely used as a phosphoramidite building block for synthesizing modified RNA in drug discovery and structural biology research.
In vitro	In RNA structural studies, 2'-O-Methyl-5-methylcytidine modification locked the ribose in the C3'-endo conformation. This modification provided steric shielding against RNase hydrolysis and increased hydrophobicity for enhanced base stacking. Collectively, these effects resulted in an increase in the melting temperature (Tm) of RNA duplexes by 0.5 to 1.3°C per modification [1].
Synonyms	5-Methyl-2′-O-methylcytidine

Chemical Properties

Molecular Weight	271.27
Formula	C₁₁H₁₇N₃O₅
Cas No.	113886-70-7
Smiles	O(C)[C@H]1[C@@H](O[C@H](CO)[C@H]1O)N2C(=O)N=C(N)C(C)=C2

Storage & Solubility Information

Storage

keep away from direct sunlight,keep away from moisture,store at low temperature | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (147.45 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.6864 mL	18.4318 mL	36.8636 mL	184.3182 mL
5 mM	0.7373 mL	3.6864 mL	7.3727 mL	36.8636 mL
10 mM	0.3686 mL	1.8432 mL	3.6864 mL	18.4318 mL
20 mM	0.1843 mL	0.9216 mL	1.8432 mL	9.2159 mL
50 mM	0.0737 mL	0.3686 mL	0.7373 mL	3.6864 mL
100 mM	0.0369 mL	0.1843 mL	0.3686 mL	1.8432 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc