β-catenin-IN-9 is a β-catenin inhibitor that induces apoptosis, causes cell cycle arrest, and suppresses the migration, invasion, and epithelial-mesenchymal transition (EMT) of colorectal cancer cells. It inhibits the transcription of β-catenin and vimentin and significantly reduces β-catenin at the protein level. β-catenin-IN-9 is applicable for research in colorectal cancer.

β-catenin-IN-9 (compound 10d) exhibits potent cytotoxic activity against Colo-205 cells with an IC50 value of 0.55 μM. At a concentration of 0.55 μM for 48 hours, β-catenin-IN-9 significantly induces apoptosis, inhibits migration and invasion, and causes cell cycle arrest in colorectal cancer cells, leading to increased overall cell death and accumulation in the S phase. Additionally, it markedly reduces β-catenin protein levels while elevating vimentin protein levels. The compound also substantially suppresses the gene expression of β-catenin and vimentin and promotes the expression of E-cadherin.