β-Amyloid (1-42), rat/mouse TFA is a 42-amino-acid polypeptide fragment that exhibits neurotoxicity to hippocampal slices and is commonly used to establish Alzheimer's disease models for related research.

β-Amyloid Aggregation Guidelines (This protocol provides only a guideline and should be modified according to your specific needs).
Dissolve the solid Aβ peptide in cold hexafluoro-2-propanol (HFIP). Incubate the peptide at room temperature for at least 1 hour to establish monomerization and randomization of the structural formation.Evaporate HFIP to preserve the obtained peptide in a film form at -20 or -80°C.Dissolve the obtained film in anhydrous DMSO (5 mM) and then vortex to dilute it to an appropriate concentration in a buffer (serum-free and phenol red-free medium).Subsequently, age the solution at 4-8°C for 48 hours. Centrifuge the samples at 14,000 g for 10 minutes at 4-8°C; soluble oligomers will be present in the supernatant. Dilute the supernatant 10-200 times for experimental use.The method may vary based on downstream applications.

DMSO: 50 mg/mL (11.03 mM), Sonication is recommended.