Loaded Recombinant Human OPG-Fc on Pro A Biosensor, can bind Mouse RANKL-His with an affinity constant of 1.02 pM as determined in BLI assay. (Regularly tested)

RANKL/TNFSF11/CD254 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 30-35 KDa and the accession number is BAA97257.1.

Mouse

HEK293 Cells

Tumor necrosis factor ligand superfamily member 11,TRANCE,Tnfsf11,TNF-related activation-induced cytokine,Receptor activator of nuclear factor κ-B ligand,Receptor activator of nuclear factor kappa-B ligand,RANKL,Osteoprotegerin ligand,Osteoclast differentiation factor,OPGL,ODF,CD254

Arg43-Asp287

Arg43-Asp287

Greater than 90% as determined by reducing SDS-PAGE. Greater than 90% as determined by SEC-HPLC.

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Mouse tumor necrosis factor ligand superfamily member 11(Tnfsf11) is a member of the tumor necrosis factor (TNF) cytokine family. Tnfsf11 is widely expressed in cells including T cells and T cell rich organs, such as thymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG andTNFRSF11A/RANK. Tnfsf11 is involved in a number of fundamental biological processes such as acting as regulator of interactions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cell proliferation.