LDLR Protein, Human, Recombinant (mFc)

Name: LDLR Protein, Human, Recombinant (mFc)
Brand: TargetMol
SKU: TMPY-05323
Rating: 4.5 (1 reviews)

(Synonyms: low density lipoprotein receptor, LDLCQ2, LDL Receptor, LDL R, FHC, FH) Copy Product Info

Synonyms: low density lipoprotein receptor, LDLCQ2, LDL Receptor, LDL R, FHC, FH

Catalog No. TMPY-05323 Copy Product Info

LDLR Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 111.2 kDa and the accession number is A0A024R7D5.

Pack Size	Price	USA Stock	Global Stock
5 µg	$79	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$208	7-10 days	7-10 days
50 µg	$419	7-10 days	7-10 days
100 µg	$812	7-10 days	7-10 days
200 µg	$1,230	7-10 days	7-10 days
500 µg	$2,530	7-10 days	7-10 days
1 mg	$5,280	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1.Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag on ProA Biosensor, can bind Recombinant Human PCSK9 (D374Y) Protein, His Tag with an affinity constant of 2.57nM as determined in BLI assay (Routinely tested). 2.Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag on ProA Biosensor, can bind Human PCSK9 / NARC1 (D374Y, V474I, G670E) Protein, His Tag with an affinity constant of 3.08nM as determined in BLI assay (Routinely tested). 3.Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag on ProA Biosensor, can bind Recombinant Human PCSK9 Protein, His Tag with an affinity constant of 16nM as determined in BLI assay (QC tested).
Description	LDLR Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 111.2 kDa and the accession number is A0A024R7D5.
Species	Human
Expression System	HEK293 Cells
Tag	C-mFc
Accession Number	P01130
Construction	A DNA sequence encoding the human LDLR (NP_000518.1) (Met1-Arg788) was expressed with the Fc region of mouse IgG1 at the C-terminus. Predicted N terminal: Ala 22
Protein Purity	> 85 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM Glycine, 10 mM NaCl, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	low density lipoprotein receptor, LDLCQ2, LDL Receptor, LDL R, FHC, FH
Research Background	LDL Receptor, also known as LDLR, is a mosaic protein that belongs to the Low-density lipoprotein receptor gene family. The low-density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low-density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.

Chemical Properties

Molecular Weight

111.2 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.