HRG/HPRG Protein, Rat, Recombinant (His)

Name: HRG/HPRG Protein, Rat, Recombinant (His)
Brand: TargetMol
SKU: TMPY-04788
Rating: 4.5 (1 reviews)

(Synonyms: histidine-rich glycoprotein) Copy Product Info

Synonyms: histidine-rich glycoprotein

Catalog No. TMPY-04788 Copy Product Info

HRG/HPRG Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.6 kDa and the accession number is A6JS43.

Pack Size	Price	EUR Stock	Global Stock
5 µg	71 €	7-10 days	7-10 days
10 µg	116 €	7-10 days	7-10 days
20 µg	187 €	7-10 days	7-10 days
50 µg	377 €	7-10 days	7-10 days
100 µg	730 €	7-10 days	7-10 days

For In stock only · Estimated delivery: EUR Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	HRG/HPRG Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.6 kDa and the accession number is A6JS43.
Species	Rat
Expression System	HEK293 Cells
Tag	C-His
Accession Number	A6JS43
Construction	A DNA sequence encoding the rat HRG (EDL78064.1) (Met1-Lys525) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 19
Protein Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	histidine-rich glycoprotein
Research Background	Histidine-rich glycoprotein, also known as HRG and HPRG, is a glycoprotein located in plasma and platelets and contains an unusually large amount of histidine and proline. In humans, five distinct domains are recognized in the mature HPRG molecule. There are two N-terminal cystatin-like modules (aa 19 - 254) and one His-Pro-rich region (aa 350 - 497) that is flanked by two Pro-rich segments (aa 276 - 321 and 498 - 525). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond. The specific functions of HRG remain unclear, but it is known that the protein binds heme, dyes, and divalent metal ions. It inhibits rosette formation and interacts with heparin, thrombospondin, and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis, and the reduction of inhibition of coagulation indicate a potential prothrombotic effect. HPRG is evolutionarily, functionally, and structurally related to cleaved high molecular weight kininogen (HKa), an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin. The antiangiogenic activity of the multidomain plasma protein HPRG is localized to its histidine-proline-rich (H/P) domain and has recently been shown to be mediated, at least partially, through binding to cell-surface tropomyosin in fibroblast growth factor-2-activated endothelial cells.

Chemical Properties

Molecular Weight

58.6 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.