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EphB2 Protein, Human, Recombinant (aa 570-987, His & GST)

Catalog No. TMPY-04386
Synonyms: PCBC, CAPB, Hek5, EK5, EPHT3, EPH receptor B2, Tyro5, DRT, ERK

EphB2 Protein, Human, Recombinant (aa 570-987, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 75.2 kDa and the accession number is P29323-3.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
EphB2 Protein, Human, Recombinant (aa 570-987, His & GST)
Pack Size Availability Price/USD Quantity
50 μg 5 days $ 498.00
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Biological Description
Technical Params
Product Properties
References and Literature
Description EphB2 Protein, Human, Recombinant (aa 570-987, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 75.2 kDa and the accession number is P29323-3.
Species Human
Expression System Baculovirus Insect Cells
Tag His, GST
Accession Number P29323-3
Synonyms PCBC, CAPB, Hek5, EK5, EPHT3, EPH receptor B2, Tyro5, DRT, ERK
Construction The Human EPHB2 (P29323-3) (Gly570-Val987) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity > 90 % as determined by SDS-PAGE
Molecular Weight 75.2 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 3 mM DTT, 10% gly.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.

Research Background Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

References and Literature

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Keywords

EphB2 Protein, Human, Recombinant (aa 570-987, His & GST) PCBC CAPB Hek5 EK5 EPHT3 EPHT 3 EPH receptor B2 EK 5 Tyro5 DRT ERK EPHT-3 EK-5 recombinant recombinant-proteins proteins protein

 

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