DPP4/CD26 Protein, Human, Recombinant

Name: DPP4/CD26 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-00779
Price: 86 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: TP103, DPPIV, dipeptidylpeptidase 4, CD26, ADCP2, ADABP) Copy Product Info

Synonyms: TP103, DPPIV, dipeptidylpeptidase 4, CD26, ADCP2, ADABP

Catalog No. TMPY-00779 Copy Product Info

DPP4/CD26 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 85.4 kDa and the accession number is P27487.

Pack Size	Price	USA Stock	Global Stock
5 µg	$86	-	In Stock
10 µg	$139	-	In Stock
20 µg	$229	-	In Stock
50 µg	$451	-	In Stock
100 µg	$771	7-10 days	7-10 days
200 µg	$1,310	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:SDS-PAGE: 97%; SEC-HPLC: 99.3%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is > 2,500 pmoles/min/μg. 2. Using the Octet RED System, the affinity constant (Kd) of human DPP4 bound to Spike (HCoV-EMC/2012) was 20 nM. 3. Using the Octet RED System, the affinity constant (Kd) of human DPP4 bound to Spike (HCoV-EMC/2012) was 50 nM. 4. Using the Octet RED System, the affinity constant (Kd) of human DPP4 bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) was 33 nM. 5. Using the Octet RED System, the affinity constant (Kd) of human DPP4 bound to Spike-His (aa 1-760) was 12 nM.
Description	DPP4/CD26 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 85.4 kDa and the accession number is P27487.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P27487
Construction	The native mature form of human DPPIV (NP_001926.2) extracellular domain (Asn 29-Pro 766) was expressed and purified. Predicted N terminal: Asn 29
Protein Purity	≥ 70 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	TP103, DPPIV, dipeptidylpeptidase 4, CD26, ADCP2, ADABP
Research Background	Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell types. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV). The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA(1c)) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating Type 2 diabetes.

Chemical Properties

Molecular Weight

85.4 kDa (predicted); 95 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.