Z164597606 is a compound that acts as a selective inhibitor of the enzyme Butyrylcholinesterase (BChE), with IC50 values of 1.3 μM and 1.7 μM for equine BChE and human BChE, respectively. Through a π-π stacking interaction, Z164597606 specifically interacts with the amino acid Trp82 of human BChE. This compound has potential applications in Alzheimer's disease research [1] [2].

eqBCHE:1.3 μM, BCHE (human):1.7 μM

Z164597606 at a concentration of 10 μM potently reduces BChE activity while demonstrating minimal inhibition (not exceeding 30%) of AChE, according to study [1]. At concentrations ranging from 0.5 to 20 μM, Z164597606 likely targets the catalytic anionic site (CAS) of BChE, as inferred from Lineweaver-Burk reciprocal plots [1]. Furthermore, Z164597606 has been shown to be non-toxic to the neuronal cell line SH-SY5Y at concentrations of 10 and 50 μM [1].