Shopping Cart
- Remove All
- Your shopping cart is currently empty
X-Gluc Dicyclohexylamine (5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt) is a reagent to detect β-glucuronidase and can be used in molecular biology experiments to mark and select the expression of target genes.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
2 mg | $30 | In Stock | |
5 mg | $42 | In Stock | |
10 mg | $65 | In Stock | |
25 mg | $98 | In Stock | |
50 mg | $156 | In Stock | |
100 mg | $280 | In Stock | |
200 mg | $327 | In Stock | |
1 mL x 10 mM (in DMSO) | $162 | In Stock |
Description | X-Gluc Dicyclohexylamine (5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt) is a reagent to detect β-glucuronidase and can be used in molecular biology experiments to mark and select the expression of target genes. |
Cell Research | Instructions I. Solution preparation 1. Stock solution: Dissolve X-Gluc Dicyclohexylamine powder in DMSO (dimethylsulfonamide) or acetone, the stock solution concentration is usually 50-100 mg/mL. 2. Working solution: Dilute the stock solution to a final concentration of 1-2 mg/mL using sodium phosphate buffer (pH 7.0), but the optimal concentration may need to be optimized depending on the application. II. Histochemical staining procedure 1. Sample preparation: Prepare plant tissues, cells or other samples to be stained. For plant tissues, samples may need to be fixed and permeabilized with a fixative such as formalin or glutaraldehyde. 2. Incubation: Incubate the prepared sample with X-Gluc working solution (1-2 mg/mL), usually at 37°C for several hours (usually 4-16 hours). The incubation time can be adjusted according to the sensitivity of the sample and the intensity of the desired staining. 3. Visualization: After the incubation period, wash the sample to remove excess substrate and it can then be observed under an optical microscope. β-Glucuronidase activity will result in the formation of an indigo-colored precipitate, resulting in a blue stain in the tissue. 4. Counterstain (optional): In some cases, a counterstain such as eosin may be used to highlight other features of the tissue or cells. |
Alias | 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt |
Molecular Weight | 603.93 |
Formula | C26H36BrClN2O7 |
Cas No. | 18656-96-7 |
Smiles | C1CCC(CC1)NC1CCCCC1.O[C@@H]1[C@@H](O)[C@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)O[C@@H]([C@H]1O)C(O)=O |
Relative Density. | 1.31g/cm3 |
Storage | keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||
Solubility Information | DMSO: 30 mg/mL (49.67 mM), Sonication is recommended. | |||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||
DMSO
|
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.