Tosylchloramide sodium trihydrate is an agent of disinfectant. It is also used as a biocide in air fresheners and deodorants.

Tosylchloramide effectively suppresses both Gram-positive bacterial growth and E. coli (gram-negative; with/without serum) proliferation. Gram-positive bacteria are diminished by 95-100% across all dosages and in the presence or absence of serum. For E. coli, reductions of 94-100% occur at antiseptic levels of 300 and 400 ppm. At a lower concentration of 200 ppm, E. coli growth is completely halted without serum and reduced by 50% when serum is present. Moreover, at concentrations of 100 and 200 ppm, cell viability exceeds 90% regardless of the experimental conditions. A brief, 3-minute exposure to a 300-ppm concentration of tosylchloramide maintains up to 70% cell viability, although extended exposure further diminishes viability. Notably, serum exposure does not influence cell viability under any tested conditions[1].

In rat tissues, significant dose-dependent DNA damage and inflammation were observed histopathologically around the terminal airways of the lung in both sexes after exposure to tosylchloramide. Additionally, a 24-hour exposure to 50 mg/L of chloramine-T was found toxic to crayfish, causing a significant energy loss evident during subsequent physical stress tests. Tosylchloramide has been noted to potentially enhance the toxicity of various xenobiotics through metabolic activation and/or the accumulation of reactive metabolites. Specifically, the activities of enzymes CYP2E1, CYP1A1/2, CYP2B1/2, CYP3A4, and CYP4A1/2 significantly increased in a dose-dependent manner with tosylchloramide doses of 2.50, 5, and 10 mg/kg bw/day. This increase in enzyme activity was not observed at a lower dose of 1.25 mg/kg bw/day.