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TMR Biocytin
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Catalog No. T87537Cas No. 749247-49-2
TMR Biocytin, a polar tracer, is utilized in researching cell-cell and cell-liposome fusions, membrane permeability, and cellular uptake during pinocytosis. Detectable with streptavidin, it serves as an effective neuronal tracer in live tissue (Ex=544 nm, Em=571 nm) [1].
TMR Biocytin
Copy Product Info😃Good
Catalog No. T87537Cas No. 749247-49-2
TMR Biocytin, a polar tracer, is utilized in researching cell-cell and cell-liposome fusions, membrane permeability, and cellular uptake during pinocytosis. Detectable with streptavidin, it serves as an effective neuronal tracer in live tissue (Ex=544 nm, Em=571 nm) [1].
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 mg | Inquiry | 3-6 months | 3-6 months | |
| 50 mg | Inquiry | 3-6 months | 3-6 months | |
| 100 mg | Inquiry | 3-6 months | 3-6 months |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | TMR Biocytin, a polar tracer, is utilized in researching cell-cell and cell-liposome fusions, membrane permeability, and cellular uptake during pinocytosis. Detectable with streptavidin, it serves as an effective neuronal tracer in live tissue (Ex=544 nm, Em=571 nm) [1]. |
| In vivo | TMR Biocytin is employed to assess the permeability changes in the blood-brain barrier (BBB) [2]. Guidelines (Following is our recommended protocol. This protocol provides a guideline and should be modified according to your specific needs) [2]: 1. Dilute 1 mg TMR Biocytin in 100 μL PBS (per mouse) and inject into the tail vein. 2. After 30 minutes, anesthetize and perfuse the animals. 3. Extract spinal cords and prepare deep-frozen, serial 10 μm longitudinal sections. 4. Stain nuclei with DAPI. 5. Capture images of entire sections at ×10 objective and ×10 eyepiece magnification, maintaining identical laser intensity, exposure times, and magnification across all cohorts. 6. These parameters were established using livers from both tracer-injected and non-injected mice. |
| Molecular Weight | 869.08 |
| Formula | C46H60N8O7S |
| Cas No. | 749247-49-2 |
| Smiles | C([O-])(=O)C1=C(C=2C3=C([O+]=C4C2C=CC(N(C)C)=C4)C=C(N(C)C)C=C3)C=CC(C(NCCCCCNC([C@H](CCCCNC(CCCC[C@H]5[C@@]6([C@](CS5)(NC(=O)N6)[H])[H])=O)N)=O)=O)=C1 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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