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SYBR GREEN 1

Name: SYBR GREEN 1
Brand: TargetMol
SKU: TD0001
Rating: 4.5 (1 reviews)

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Catalog No. TD0001Cas No. 163795-75-3

Alias Green-DNA Dye,SYBR Green I

SYBR Green Ⅰ (Green-DNA Dye, SYBR Green I) is a dye that binds to all dsDNA double helix minor groove regions, with a maximum absorption wavelength around 497nm and a maximum emission wavelength around 520nm.

SYBR GREEN 1

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Catalog No. TD0001Alias Green-DNA Dye,SYBR Green ICas No. 163795-75-3

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
100 μL	$218	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Liquid

Color:Transparent to Red

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COA

Product Introduction

Bioactivity

Description	SYBR Green Ⅰ (Green-DNA Dye, SYBR Green I) is a dye that binds to all dsDNA double helix minor groove regions, with a maximum absorption wavelength around 497nm and a maximum emission wavelength around 520nm.
In vitro	Precasting SYBR Green I Gels:Precast nondenaturing polyacrylamide or agarose gels with SYBR Green I staining solution, which is diluted from the SYBR Green I stock reagent 1:10,000 prior to pouring the gel. The DNA detection limit for gels precast with SYBR Green I stain may be slightly higher (on the order of 30 to 40 pg/band) than for gels stained after electrophoresis (less than 20 pg/band), and the migration rate of DNA fragments in SYBR Green I gels may be significantly slower than the migration rate of the same fragments in a gel containing no dye.
Synonyms	Green-DNA Dye,SYBR Green I

Chemical Properties

Molecular Weight	509.73
Formula	C₃₂H₃₇N₄S
Cas No.	163795-75-3
Smiles	S1C=2C=CC=CC2N(C1=CC3=CC(N(CCC)CCCN(C)C)=[N+](C=4C=CC=CC4)C=5C=CC=CC35)C
Relative Density.	no data available

Storage & Solubility Information

Storage

keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

SYBR(R) GREEN I NUCLEIC ACID GEL STAIN *10,000× concentrate in DMSOSYBR GREEN 1