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PF-AKT400

Name: PF-AKT400
Brand: TargetMol
SKU: T5508
Price: 96 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T5508Cas No. 1004990-28-6

Alias AKT protein kinase inhibitor

PF-AKT400 (AKT protein kinase inhibitor) is a broadly selective and ATP-competitive inhibitor of Akt.

PF-AKT400

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Purity: 98.21%

Catalog No. T5508Alias AKT protein kinase inhibitorCas No. 1004990-28-6

PF-AKT400 (AKT protein kinase inhibitor) is a broadly selective and ATP-competitive inhibitor of Akt.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$96	In Stock	-
5 mg	$239	In Stock	-
10 mg	$343	In Stock	-
25 mg	$579	In Stock	-
50 mg	$829	In Stock	-
100 mg	$1,150	Inquiry	Inquiry
1 mL x 10 mM (in DMSO)	$263	In Stock	-

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:98.21%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

PF-AKT400 AI Summary

PF-AKT400 shows potent bioactivity based on multiple assays. It exhibits high oral bioavailability in various animal models, such as dogs and rats, and excellent inhibitory activity against AKT1 and AKT2 in IMAP assays, with IC50 values of 0.5 nM and 9.0 nM, respectively. The compound demonstrates selectivity towards AKT1 over other kinases like RSK3, RSK2, PKG1alpha, MSK2, MSK1, and CDK7. Additionally, it shows antitumor activity against human PC3 and COLO205 cells xenografted in SCID/Beige mice, resulting in significant tumor growth inhibition. PF-AKT400 effectively inhibits the AKT1/p70S6K1 signaling pathways, leading to reduced phosphorylation levels of S6 protein, and exhibits strong AKT1/p70S6K1 inhibition in both single-dose and multiple-dose administrations, as well as feedback loop-associated activities. These data suggest that PF-AKT400 has promising potential as a candidate for further development as an anticancer therapy targeting the AKT signaling pathway..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	PF-AKT400 (AKT protein kinase inhibitor) is a broadly selective and ATP-competitive inhibitor of Akt.
Targets&IC50	PKBα:0.5 nM, PKA:450 nM
Synonyms	AKT protein kinase inhibitor

Chemical Properties

Molecular Weight	400.43
Formula	C₂₀H₂₂F₂N₆O
Cas No.	1004990-28-6
Smiles	CCc1c[nH]c2ncnc(N3CC[C@](N)(CNC(=O)c4ccc(F)cc4F)C3)c12
Relative Density.	1.353 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 90 mg/mL (224.76 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (8.24 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4973 mL	12.4866 mL	24.9732 mL	124.8658 mL
5 mM	0.4995 mL	2.4973 mL	4.9946 mL	24.9732 mL
10 mM	0.2497 mL	1.2487 mL	2.4973 mL	12.4866 mL
20 mM	0.1249 mL	0.6243 mL	1.2487 mL	6.2433 mL
50 mM	0.0499 mL	0.2497 mL	0.4995 mL	2.4973 mL
100 mM	0.0250 mL	0.1249 mL	0.2497 mL	1.2487 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc