Octadecyl Rhodamine B chloride, also known as RBOE, is a polarity-sensitive dye that is often used as a chromophore with other compounds to stain cell membranes and has applications in the assembly of organic molecules, in membrane structures and in the transfer of electron energy in the structural domains of proteins.

Octadecyl Rhodamine B Chloride virus infection cell experiment
a. Solution preparation:
1. Mother solution preparation: Prepare a certain concentration of Dansylcadaverine mother solution, store it at -20℃ or -80℃ in the dark after aliquoting.
2. Working solution preparation: Select the appropriate working solution concentration according to the experimental requirements, and try to prepare it for immediate use.
b. Operation steps:
1. Octadecyl Rhodamine B Chloride is incubated at 4°C with rotation for 8 hours according to the ratio of dye: virus 1:500 (volume ratio), and then filtered with a 0.22 μM filter for standby use.
2. Spread the well-growing cells in a laser confocal dish, wait until the cell confluence reaches about 80%, and infect the cells with influenza virus labeled with Octadecyl Rhodamine B Chloride, adsorb at 4°C for 1 hour, and culture at 37°C for 30 min, 60 min, 90 min and 120 min.
3. Detect the fluorescence intensity and fluorescence localization in cells by laser confocal microscopy.