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NFQ1, a non-fluorescent quencher (absorption wavelength: 474 nm), is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method, enabling measurement of unquenched donor emission in the same sample. NFQ1 shows a broad absorption spectrum, ensuring its utility as a dark acceptor for many donors [1].

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| Description | NFQ1, a non-fluorescent quencher (absorption wavelength: 474 nm), is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method, enabling measurement of unquenched donor emission in the same sample. NFQ1 shows a broad absorption spectrum, ensuring its utility as a dark acceptor for many donors [1]. |
| In vitro | NFQ1 is used for the One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method, employing a novel, non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond. After quantitating the quenched donor emission, the acceptor is removed by reduction, enabling measurement of unquenched donor emission in the same sample [1]. |
| Molecular Weight | 451.48 |
| Formula | C17H17N5O6S2 |
| Cas No. | 1262391-07-0 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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