LasR-IN-3, a chemical compound, acts as an inhibitor of LasR in Pseudomonas aeruginosa. It induces structural instability in LasR and causes complete dissociation of its functioning dimeric form [1].

LasR-IN-3 (compound 7f), ranging from 4.68 to 150 μg/mL over 24 hours, demonstrates anti-Pseudomonas aeruginosa activity, with Minimum Inhibitory Concentration (MIC) and sub-MIC levels recorded at 56.25 μg/mL and 14.00 μg/mL, respectively. At a concentration of 14.00 μg/mL for either 24 or 48 hours, it effectively inhibits biofilm formation and the production of pyocyanin and rhamnolipids by 71.70%, 68.70%, and 54.00%, respectively. Additionally, LasR-IN-3 is proven safe for normal HDFa cell lines at concentrations of 56.25 μg/mL and 338.0 μg/mL over 24 hours. At 14.00 μg/mL for 24 hours, it also reduces the expression of the lasR and rhlR genes, thereby impeding QS-dependent biofilm formation and virulence factors. RT-PCR results confirm a decrease in lasR and rhlR gene expression by 26% and 16.3%, respectively, at a concentration of 14.00 μg/mL after 24 hours.