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IR-820

🥰Excellent
Catalog No. T20870Cas No. 172616-80-7
Alias New Indocyanine Green, IR820, IR 820

IR-820 (New Indocyanine Green) is a near-infrared dye with improved stability, good color rendering, and excellent fluorescence penetration. It serves as an infrared blood pool contrast agent for detecting and quantifying diseased tissue in live animals, with maximal excitation (Ex) and emission (Em) of 710 and 820 nm, respectively.

IR-820

IR-820

🥰Excellent
Purity: 98.76%
Catalog No. T20870Alias New Indocyanine Green, IR820, IR 820Cas No. 172616-80-7
IR-820 (New Indocyanine Green) is a near-infrared dye with improved stability, good color rendering, and excellent fluorescence penetration. It serves as an infrared blood pool contrast agent for detecting and quantifying diseased tissue in live animals, with maximal excitation (Ex) and emission (Em) of 710 and 820 nm, respectively.
Pack SizePriceAvailabilityQuantity
50 mg$30In Stock
100 mg$39In Stock
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Purity:98.76%
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Product Introduction

Bioactivity
Description
IR-820 (New Indocyanine Green) is a near-infrared dye with improved stability, good color rendering, and excellent fluorescence penetration. It serves as an infrared blood pool contrast agent for detecting and quantifying diseased tissue in live animals, with maximal excitation (Ex) and emission (Em) of 710 and 820 nm, respectively.
In vitro
Instructions:
I. Solution preparation
1. Stock solution: Prepare 1–10 mM stock solution, dissolve in sterile water or DMSO, and adjust the concentration according to experimental needs.
2. Working concentration: Dilute the stock solution to 10–50 µM (in vitro) or 0.5–1 mg/kg (in vivo) according to the experimental objectives.
Notes:
Powder: It is recommended to store in a dry environment away from light, and the temperature is controlled at -20°C or lower.
Solution: It can be stored at 4°C for short-term storage, and it needs to be aliquoted and kept away from light for long-term storage, and stored at -20°C to avoid repeated freezing and thawing.
II. Bioimaging experiment
IR820 is commonly used in near-infrared fluorescence imaging (NIRF) and is suitable for in vivo or in vitro imaging studies.
Experimental steps:
In vitro experiment: Directly add IR820 working solution to the cell culture system and incubate for 30–60 minutes.
In vivo experiment: Dilute IR820 and inject it into the experimental animal through the tail vein.
The signal is detected by near-infrared imaging equipment during imaging.
III. Photothermal/photodynamic therapy
IR820 has photothermal and photosensitivity properties and can be used for photothermal therapy or photodynamic therapy of tumors.
Illumination conditions: Use 808 nm laser irradiation, and the light intensity and time are optimized according to the experiment (such as 1–2 W/cm², 5–10 minutes).
Concentration reference: Dilute IR820 to 5–20 µM (in vitro) or 0.5–2 mg/kg (in vivo) according to the treatment goal.
IV. Fluorescence detection parameters
Excitation wavelength: about 700–800 nm
Emission wavelength: about 820–850 nm
Precautions
Light-avoiding operation: IR820 is sensitive to light, and strong light should be avoided during the operation.
Fresh preparation: It is recommended to prepare it before use to prevent degradation of the dissolving solution.
Control: A blank group and a control group should be set up in the experiment to ensure reliable data.
AliasNew Indocyanine Green, IR820, IR 820
Chemical Properties
Molecular Weight849.47
FormulaC46H50ClN2NaO6S2
Cas No.172616-80-7
Smiles[Na+].CC1(C)C(=CC=C2CCCC(C=CC3=[N+](CCCCS([O-])(=O)=O)c4ccc5ccccc5c4C3(C)C)=C2Cl)N(CCCCS([O-])(=O)=O)c2ccc3ccccc3c12
Relative Density.no data available
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 4.5 mg/mL (5.3 mM), Sonication and heating to 60℃ are recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.1772 mL5.8860 mL11.7720 mL58.8602 mL
5 mM0.2354 mL1.1772 mL2.3544 mL11.7720 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
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2 Enter the in vivo formulation:
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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