IR-780 Iodide (IR 780 iodide), a near‐infrared fluorescent dye, is used for the exclusive characterization of human CSCs through the HIF‐1α/glycolysis dependent mitochondrial transporter ABCB10's activity.

1. For cancer stem cell (CSC) research
1. Cell staining: Dissolve IR-780 Iodide in an appropriate solvent (such as PBS or DMEM culture medium), usually at a concentration of 10-20 μM. Add the dye solution to the cell culture medium and incubate for 30-60 minutes.
2. Washing: After staining, gently wash the cells with PBS to remove unbound dye.
3. Fluorescence microscopy: Observe using a near-infrared fluorescence microscope (e.g., excitation wavelength 745 nm, emission wavelength 780 nm). IR-780 Iodide can be localized within the cell and its distribution in different subcellular structures, especially mitochondria and cell membrane regions, can be observed.
2. For HIF-1α pathway research
1. Cell treatment: Select a suitable cancer cell line or CSC, add IR-780 Iodide and incubate, and observe the adsorption of the dye and its correlation with mitochondrial transport proteins.
2. Fluorescence detection: Fluorescence imaging technology is used to detect the effect of changes in HIF-1α pathway activity on the distribution and metabolic pathway of IR-780 dye.
III. Used for multidrug resistance research
1. Staining and treatment: Add IR-780 Iodide dye to drug-treated cells to study the effect of drugs on the distribution of intracellular dye.
2. Flow cytometry detection: Use flow cytometry to analyze cells, study the binding effects of different drugs and IR-780, and evaluate the activity of transporters.
IV. Cell imaging and positioning
1. Staining steps: Add IR-780 Iodide solution to cell culture medium and incubate for a certain period of time (usually 30 minutes to 1 hour).
2. Microscopic imaging: Use a high-resolution near-infrared fluorescence microscope to observe cell staining and fluorescence distribution.