Hepcidin-1 (mouse) TFA, an endogenous peptide hormone, regulates iron homeostasis by upregulating mRNA levels of TRAP, cathepsin K, and MMP-9, enhancing TRAP-5b protein secretion, and downregulating FPN1 protein levels, thereby increasing intracellular iron. It also promotes osteoclast differentiation [1].

Hepcidin-1 (mouse) TFA, administered at concentrations ranging from 200 to 800 nM for four days, enhances the differentiation of RAW264.7 cells induced by RANKL at 50 ng/mL [1]. Within the same dosage range and duration, it upregulates the expression of TRAP, CTK, and MMP-9 mRNA [1]. Additionally, treatment with Hepcidin-1 (mouse) TFA at 0-800 nM for 20 hours increases the level of Trap-5b protein and decreases the ferroportin 1 (FPN1) protein in RAW264.7 cells [1].