Diphenylterazine (DTZ) is a bioluminescent agent. It produces very little background of its own, giving it an excellent signal-to-noise ratio.

Diphenylterazine alone yields very little background with excellent signal-to-background ratios. Furthermore, Diphenylterazine elicits minimal cell toxicity at millimolar concentrations. Diphenylterazine injections into untransfected BALB/c mice do not yield any background emission.

I. Solution preparation
1. Preparation of mother solution: Dissolve DTZ in DMSO or ethanol to prepare a 1-10 mM mother solution; (It is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting)
2. Preparation of working solution: Dilute the DTZ mother solution with PBS or other suitable buffer, usually 1-10 µM; (Select the appropriate working solution concentration according to the experimental requirements, and prepare it for immediate use)
Imaging analysis experiment of Staphylococcus aureus reporter strain colonization in deep tissues of mice
Operation steps: 24 hours after infection with CFU reporter strain, 1 μmol Diphenylterazine was injected intraperitoneally into the abdominal cavity of infected mice, and the mice were placed in a small animal in vivo imager to anesthetize them. After 3 minutes, the luminescent signal of the dorsal kidney of the mice was detected using the IVIS® Lumina LT system.