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DASPEI

Catalog No. T78456 CAS 3785-01-1

DASPEI is a cationic styrenyl mitochondrial dye characterized by a large Stokes shift and excitation/emission wavelengths at 550/573 nm, yielding robust chromogenic properties. It effectively stains mitochondria in live cells and labels presynaptic nerve endings regardless of neuronal activity [1].

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DASPEI, CAS 3785-01-1

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Biological Description

Chemical Properties

Storage & Solubility Information

Description

In vitro

Preparation of DASPEI Working Solution: Dissolve DASPEI in DMSO to create a 10 mM stock solution, which should be aliquoted and stored at -20°C or -80°C, protected from light. Dilute the stock with pre-warmed serum-free cell culture medium or PBS to a final concentration of 5-10 μM immediately before use. Adjust the concentration as needed for specific applications.

Cell Staining for Suspension Cells: Collect cells by centrifugation and wash twice with PBS for 5 minutes each, targeting a cell density of 1×10^6/mL. Add 1 mL of DASPEI working solution and incubate at room temperature for 30-60 minutes. Centrifuge at 400 g for 3-4 minutes, discard the supernatant, and wash the pellet twice with PBS for 5 minutes each. Finally, resuspend cells in 1 mL of serum-free medium or PBS for observation with a fluorescence microscope or flow cytometer.

Cell Staining for Adherent Cells: Culture adherent cells on sterile coverslips. After culturing, transfer the coverslip from the medium, remove excess medium, and apply 100 μL of staining working solution. Gently agitate to ensure complete coverage and incubate for 30-60 minutes. Remove the staining solution, wash coverslips with culture medium 2-3 times for 5 minutes each, and observe using a fluorescence microscope.

Molecular Weight	380.27
Formula	C17H21IN2
CAS No.	3785-01-1

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
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The answer of 19.713 mg appears in the Mass box

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The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

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An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
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The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

DASPEI 3785-01-1 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.