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CCT244747

Name: CCT244747
Brand: TargetMol
SKU: T14904
Price: 69 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T14904Cas No. 1404095-34-6

Alias CCT 244747

CCT244747 is a potent and highly selective CHK1 inhibitor that is orally active, ATP-competitive, and cytotoxic, abrogating drug-induced S and G2 blockade and inducing apoptosis in multiple tumor cell lines.

CCT244747

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Purity: 99.17%

Catalog No. T14904Alias CCT 244747Cas No. 1404095-34-6

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$69	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.17%

Appearance:Solid

Color:Yellow

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Product Introduction

Bioactivity

Description	CCT244747 is a potent and highly selective CHK1 inhibitor that is orally active, ATP-competitive, and cytotoxic, abrogating drug-induced S and G2 blockade and inducing apoptosis in multiple tumor cell lines.
Targets&IC50	Chk1:7.7 nM
In vitro	Methods: HT29 and SW620 colon cancer cell lines were treated with CCT244747 (0.01, 0.05, 0.1, 0.5, 1, 2, 5 μM) to characterize its effects on drug-induced changes in CHK1 and cell cycle biomarkers. Results CCT244747 inhibited SN38- and gemcitabine-induced CHK1 activity in tumor cells, and this was associated with abrogation of cell cycle arrest, induction of DNA damage, and apoptosis. [4]
In vivo	Methods: Athymic mice bearing SW620 human colon cancer xenografts were treated with CCT244747 (100-300 mg/kg, oral) followed by gemcitabine (60 mg/kg, intravenous) 1 hour later to further analyze the pharmacodynamics of CCT244747. Results: A single oral dose of CCT244747 (100-300 mg/kg) maintained inhibition of gemcitabine-induced pS296 CHK1 in HT29 colon tumor xenografts for up to 24 hours. [1]
Synonyms	CCT 244747

Chemical Properties

Molecular Weight	408.46
Formula	C₂₀H₂₄N₈O₂
Cas No.	1404095-34-6
Smiles	O(C)C=1C(=CN=C(NC2=NC(O[C@@H](CN(C)C)C)=C(C#N)N=C2)C1)C=3C=NN(C)C3
Relative Density.	1.27 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (97.93 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.9 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4482 mL	12.2411 mL	24.4822 mL	122.4110 mL
5 mM	0.4896 mL	2.4482 mL	4.8964 mL	24.4822 mL
10 mM	0.2448 mL	1.2241 mL	2.4482 mL	12.2411 mL
20 mM	0.1224 mL	0.6121 mL	1.2241 mL	6.1206 mL
50 mM	0.0490 mL	0.2448 mL	0.4896 mL	2.4482 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc