Calcium disodium edetate hydrate

Calcium disodium edetate hydrate possesses both metal-chelating and antifungal properties. Calcium disodium edetate hydrate chelates Mn²⁺, causing mitochondrial damage, disrupting carbohydrate metabolic pathways, and inhibiting the production of pyruvate during glycolysis. It inhibits the growth of Penicillium digitatum, delays conidial germination, and enhances the innate defense capabilities of citrus fruits, making it suitable for research into the mechanisms underlying citrus green mold disease.

Methods: A concentration gradient of 3.125–50 mmol/L Calcium disodium edetate hydrate was established. The Penicillium digitatum P44 strain was treated in vitro, and hyphal growth was observed while MIC and MFC were determined; Exposure to drug solutions ranging from 12.5 to 50 mmol/L for 8–12 h was used to evaluate the inhibitory effect on conidial germination of the strain.
Results: Calcium disodium edetate hydrate completely inhibited the in vitro growth of Penicillium digitatum P44 hyphae; both the MIC and MFC were 50 mmol/L. Under conditions of 12.5–50 mmol/L and an exposure time of 8–12 h, it dose-dependently delayed and inhibited the germination of conidia from this strain [2].

Methods: Plants infected with citrus green mold were treated via intra-lesional injection with 25–250 mmol/L Calcium disodium edetate hydrate. The disease incidence and lesion size were observed and recorded.
Results: Intralesional injection of Calcium disodium edetate hydrate reduced the incidence of citrus green mold in a dose-dependent manner, while significantly inhibiting lesion expansion and reducing lesion area [2].

DMSO: 100 mg/mL, Sonication is recommended.