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BP Fluor 532 NHS ester is an amine-reactive yellow fluorescent dye commonly employed for labeling proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling process is most effective at pH 7-9, resulting in the formation of stable covalent amide bonds. BP Fluor 532 exhibits bright yellow fluorescence that is insensitive to pH changes from 4 to 10 and is optimally excited by the Nd:YAG laser line. It can bind with a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is frequently used to produce stable signals in imaging and flow cytometry.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | 10-14 weeks | 10-14 weeks | |
| 50 mg | Inquiry | 10-14 weeks | 10-14 weeks |
| Description | BP Fluor 532 NHS ester is an amine-reactive yellow fluorescent dye commonly employed for labeling proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling process is most effective at pH 7-9, resulting in the formation of stable covalent amide bonds. BP Fluor 532 exhibits bright yellow fluorescence that is insensitive to pH changes from 4 to 10 and is optimally excited by the Nd:YAG laser line. It can bind with a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is frequently used to produce stable signals in imaging and flow cytometry. |
| Synonyms | 477876-64-5 |
| Molecular Weight | 723.769 |
| Formula | C34H33N3O11S2 |
| Cas No. | 2791287-15-3 |
| Smiles | O=C(ON1C(=O)CCC1=O)C=2C=CC(=CC2)C=3C=4C=C5C(NC(C)C5(C)C)=C(C4[O+]=C6C3C=C7C(NC(C)C7(C)C)=C6S(=O)(=O)O)S(=O)(=O)[O-] |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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