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Bis(4-nitrophenyl) phosphate

Name: Bis(4-nitrophenyl) phosphate
Brand: TargetMol
SKU: T65012
Price: 40 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T65012Cas No. 645-15-8

Alias BNPP

Bis(4-nitrophenyl) phosphate (BNPP) inhibits UDP-galactose and can be used to study glycosyltransferase membrane topology.

Bis(4-nitrophenyl) phosphate

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Purity: 99.92%

Catalog No. T65012Alias BNPPCas No. 645-15-8

Bis(4-nitrophenyl) phosphate (BNPP) inhibits UDP-galactose and can be used to study glycosyltransferase membrane topology.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
500 mg	$40	In Stock	-
1 g	$64	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.92%

Appearance:Solid

Color:White

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COA HPLC HNMR

Product Introduction

Bis(4-nitrophenyl) phosphate AI Summary

Bis(4-nitrophenyl) phosphate exhibits multiple bioactivities across different biological targets. It acts as an inhibitor of the Rango-Importin beta complex formation with a potency of 12589.3 nM and also inhibits RanGTP-induced Rango-Importin beta complex dissociation with a potency of 18356.4 nM. Additionally, the compound shows agonistic activity towards gsp, involved in Fibrous Dysplasia/McCune-Albright Syndrome, with a potency of 7079.5 nM. In terms of enzyme inhibition, Bis(4-nitrophenyl) phosphate demonstrates strong inhibition of various carboxylesterases. It inhibits human carboxylesterase 1 (CE1) in liver microsomes with an IC50 value of 140.0 nM and human carboxylesterase 2 (CE2) with an IC50 value of 6110.0 nM. It also displays inhibitory activity against porcine liver carboxylesterase with an IC50 value of 1800.0 nM using 4-nitrophenol acetate as a substrate. Other enzyme targets include DLODP in human HepG2 microsomal membrane (IC50 of 1200000.0 nM) and SARS-CoV-2 3CL-Pro protease, where it shows 22.7% inhibition at 20µM. Additionally, Bis(4-nitrophenyl) phosphate exhibits antiviral activity against SARS-CoV-2 by reducing induced cytotoxicity in VERO-6 cells at a 10 µM concentration..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Bis(4-nitrophenyl) phosphate (BNPP) inhibits UDP-galactose and can be used to study glycosyltransferase membrane topology.
Synonyms	BNPP

Chemical Properties

Molecular Weight	340.18
Formula	C₁₂H₉N₂O₈P
Cas No.	645-15-8
Smiles	OP(=O)(Oc1ccc(cc1)[N+]([O-])=O)Oc1ccc(cc1)[N+]([O-])=O

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (293.96 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9396 mL	14.6981 mL	29.3962 mL	146.9810 mL
5 mM	0.5879 mL	2.9396 mL	5.8792 mL	29.3962 mL
10 mM	0.2940 mL	1.4698 mL	2.9396 mL	14.6981 mL
20 mM	0.1470 mL	0.7349 mL	1.4698 mL	7.3491 mL
50 mM	0.0588 mL	0.2940 mL	0.5879 mL	2.9396 mL
100 mM	0.0294 mL	0.1470 mL	0.2940 mL	1.4698 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc