Biotin-PEG-Thiol (MW 2000) is a compound composed of biotin, polyethylene glycol and thiol, an active polymer that can be used to study nanomaterials.

Biotin-PEG-Thiol (MW 2000) as a functionalized ligand experiment
1. In Tris buffer (10 mM, pH 8.5), the substrate coated with poly(Tyr-CB) membrane was immersed in an aqueous solution of biotin-polyethylene glycol-thiol (1 mM) to immobilize biotin under mild alkaline conditions.
2. After immersion at room temperature for 24 h, the biotin-functionalized substrate was rinsed with PBS and placed in a fresh PBS solution.