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Biotin NHS

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Catalog No. T14579Cas No. 35013-72-0
Alias NHS-Biotin, Biotin-NHS, Biotin N-hydroxysuccinimide ester

Biotin NHS (NHS-Biotin) is an amino reactive biotin reagent used to prepare biotinylated surfaces or polypeptides.

Biotin NHS

Biotin NHS

😃Good
Purity: 99.15%
Catalog No. T14579Alias NHS-Biotin, Biotin-NHS, Biotin N-hydroxysuccinimide esterCas No. 35013-72-0
Biotin NHS (NHS-Biotin) is an amino reactive biotin reagent used to prepare biotinylated surfaces or polypeptides.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 mg$33In StockIn Stock
100 mg$45In StockIn Stock
500 mg$98-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Batch Information

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Purity:99.15%
Appearance:Solid
Color:White
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Product Introduction

Bioactivity
Description
Biotin NHS (NHS-Biotin) is an amino reactive biotin reagent used to prepare biotinylated surfaces or polypeptides.
SynonymsNHS-Biotin, Biotin-NHS, Biotin N-hydroxysuccinimide ester
Chemical Properties
Molecular Weight341.38
FormulaC14H19N3O5S
Cas No.35013-72-0
SmilesO=C(CCCCC1SCC2NC(=O)NC12)ON1C(=O)CCC1=O
Relative Density.1.45g/cm3
Storage & Solubility Information
Storagestore at low temperature | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 60 mg/mL (175.76 mM), Sonication is recommended.
In Vivo Formulation
10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.86 mM), Sonication is recommended.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.9293 mL14.6464 mL29.2929 mL146.4644 mL
5 mM0.5859 mL2.9293 mL5.8586 mL29.2929 mL
10 mM0.2929 mL1.4646 mL2.9293 mL14.6464 mL
20 mM0.1465 mL0.7323 mL1.4646 mL7.3232 mL
50 mM0.0586 mL0.2929 mL0.5859 mL2.9293 mL
100 mM0.0293 mL0.1465 mL0.2929 mL1.4646 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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