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Beta-Actin Loading Control Antibody (4L34)
😃Good
Catalog No. TMAY-00016
Beta-Actin Loading Control Antibody (4L34) is a Mouse antibody targeting Beta-Actin. Beta-Actin Loading Control Antibody (4L34) can be used in WB,IP.
Beta-Actin Loading Control Antibody (4L34)
Beta-Actin Loading Control Antibody (4L34)
😃Good
Catalog No. TMAY-00016
Beta-Actin Loading Control Antibody (4L34) is a Mouse antibody targeting Beta-Actin. Beta-Actin Loading Control Antibody (4L34) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $68 | 7-10 days | |
| 100 μL | $139 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, IP Reactivity: Human |
| Ig Type | Mouse IgG2a |
| Clone | 4L34 |
| Reactivity | Human |
| Verified Activity | 1. Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: NIH3T3 Whole Cell Lysate. -Lane C: RAW264.7 Whole Cell Lysate. -Lane D: Mouse slpeen tissue Lysate. -Lane E: Mouse lung tissue Lysate. -Lane F: Mouse brain tissue Lysate. -Lane G: C6 whole cell Lysate. -Lane H: PC-12 whole cell Lysate. -Lane I: Rat liver tissue Lysate. -Lane J: Rat lung tissue Lysate. -Lane K: Rat brain tissue lysate. -Lysates/proteins at 30 μg per lane. -Secondary -HRP Conjugated Goat anti-Mouse IgG (H+L) , at 1:10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:43 kDa. -Observed band size:44 kDa. 2. Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution. -Lane A: HeLa Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: MCF7 Whole Cell Lysate. -Lane D: A431 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:43 kDa. -Observed band size:44 kDa. 3. ACTB was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate -0.5 µL anti-ACTB mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-ACTB mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 42 kDa. -Observed band size: 42 kDa. 4. Anti-beta-Actin mouse monoclonal antibody at 1:10000, 1:50000, 1:100000, 1:200000 dilution -Lysates/proteins per lane -HepG2 (30 μg), Hela (5 μg), Raw246.7 (30 μg) -Secondary -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 43 kDa. -Observed band size: 44 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:2500-1:200000; IP: 1-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the N-terminus of the Human beta Actin / ACTB. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: N-terminus of the Human beta Actin / ACTB |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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