Compound B14 (Antibacterial agent 141) exhibits antibacterial activity against the plant pathogens Xoo, Xac, Psa, and Cmm, with an EC50 value of 1.28 μM. It functions by inhibiting cell membrane formation and altering cell permeability.

Antibacterial agent 141 significantly induces cell membrane rupture in Xoo bacteria [1]. It increases membrane permeability in a concentration-dependent manner within Xoo bacteria [1], and disrupts the surface of the bacterial cell membrane [1]. At concentrations of 3.2 and 6.4 μM, the agent notably reduces the expression of ACC, ACP, and Fab family genes in Xoo bacteria [1]. Additionally, it significantly inhibits biofilm formation of Xoo with increasing concentrations [1], while exhibiting low cytotoxicity towards HepG2 and HK-2 cells [1]. At concentrations ranging from 0.64 to 5.12 μM over 3 to 30 hours, it markedly suppresses the growth of Xoo bacteria [1].

Antibacterial agent 141 at a concentration of 200 μg/mL, administered as a single dose over a period of 7 days, exhibited treatment and preventive activity against bacterial blight of 4.64% and 43.33%, respectively [1].