Anti-Mouse IFN gamma Antibody (H22) is an IFN-γ-targeting inhibitory antibody raised in Armenian hamsters against mouse IFN-γ that interferes with the specific binding of ¹²⁵I-rIFN-γ to macrophages, exerts an inhibitory effect on macrophage-activating factor (MAF) activity and IFN-γ-dependent antiviral activity with an ID₅₀ of 56 ng and 2 ng, respectively, and is also capable of neutralizing endogenous IFN-γ in mice. H22 antibody is commonly employed in research on inflammatory and infectious diseases, including the pathological mechanism investigation of arthritis.

IFN-γ:2 ng (ID₅₀), IFN-γ:56 ng (ID₅₀)

Incubation with Anti-Mouse IFN gamma Antibody (H22) for 1 h potently inhibited macrophage-activating factor (MAF) activity and antiviral effects, with an ID₅₀ of 56 ng and 2 ng, respectively[4].
Treatment with Anti-Mouse IFN gamma Antibody (H22) at an ID₅₀ of 50 ng for 2 h effectively abrogated the specific binding of ¹²⁵I-rIFN gamma to macrophages[4].

Administration of Anti-Mouse IFN gamma Antibody (H22) at a dose of 200 μg on days 0, 4, 11, and 18 (5 total administrations) neutralized IFN gamma in mice[1].
Administration of Anti-Mouse IFN gamma Antibody (H22) at 250 μg on days 0, 6, 13, 21, 28, 35, and 40 (7 total administrations) significantly increased the number of arthritic limbs in a collagen-induced arthritis mouse model[2].
Intraperitoneal injection of Anti-Mouse IFN gamma Antibody (H22) at 100 μg, initiated 5 hours prior to cell transplantation and administered once weekly, completely abrogated the protective effect of Lyt-2⁺ immune T cells against Toxoplasma gondii infection in mice[3].

H22

Mouse

Monoclonal

Unconjugated