Anti-TLR4 Polyclonal Antibody 4

Anti-TLR4 Polyclonal Antibody 4 is a Rabbit antibody targeting TLR4. Anti-TLR4 Polyclonal Antibody 4 can be used in FCM, WB.

IgG

Human,Rat (predicted:Mouse,Pig,Cow,Sheep)

1. Sample:
Lane 1: H9C2 (Human) Cell Lysate at 30 μg
Lane 2: K562 (Human) Cell Lysate at 30 μg
Primary: Anti-TLR4 (TMAB-01857) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
2. Blank control: K562. Primary Antibody (green line): Rabbit Anti-TLR4 antibody (TMAB-01857)
Dilution: 0.5 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human TLR4

Human

TLR4

Toll-like receptor 4

Inflammatory mediators,Regulatory T Cells,Receptors,Macrophage / Inflamm.,TLR Signaling,Host Virus Interaction

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.