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Anti-TLR4 Polyclonal Antibody 4
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Catalog No. TMAB-01857
Anti-TLR4 Polyclonal Antibody 4 is a Rabbit antibody targeting TLR4. Anti-TLR4 Polyclonal Antibody 4 can be used in WB,FCM.
Anti-TLR4 Polyclonal Antibody 4
Anti-TLR4 Polyclonal Antibody 4
😃Good
Catalog No. TMAB-01857
Anti-TLR4 Polyclonal Antibody 4 is a Rabbit antibody targeting TLR4. Anti-TLR4 Polyclonal Antibody 4 can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,FCM Reactivity: Human (predicted:Mouse,Rat,Pig,Cow,Sheep) |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Pig,Cow,Sheep) |
| Verified Activity | 1. Sample: Lane 1: H9C2 (Human) Cell Lysate at 30 μg Lane 2: K562 (Human) Cell Lysate at 30 μg Primary: Anti-TLR4 (TMAB-01857) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kDa Observed band size: 110 kDa 2. Blank control: K562. Primary Antibody (green line): Rabbit Anti-TLR4 antibody (TMAB-01857) Dilution: 0.5 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| Application | WB,FCM |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The Toll-like receptor 4 by activating natural immunity, specific immune response involved in the start-up, Toll-like receptor 4 as an important signal transduction transmembrane receptor involved in the toxin-induced inflammation in the pathological process, its mechanisms of control On a growing concern. all regions were either double-stranded or sequenced with an alternatechemistry or covered by high quality data(i.e., phred quality >=30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regions were covered by at least one subclone; and the assembly was confirmed by restriction digest, except on the rare occasion of the clone being a YAC. The following abbreviations are used to associate primary accession numbers given in the feature table with their source databases: Em:, EMBL; Sw:, SWISSPROT; Tr:, TREMBL; Wp:, WORMPEP; Information on the WORMPEP database can be found at. TLR-4 plays an important role in microvascular leakage and leukocyte adhesion under the inflammatory condition associated with nonseptic thermal injury. |
| Immunogen | KLH conjugated synthetic peptide: human TLR4 |
| Antigen Species | Human |
| Gene ID | 7099 |
| Uniprot ID | |
| Biology Area | Inflammatory mediators,Regulatory T Cells,Receptors,Macrophage / Inflamm.,TLR Signaling,Host Virus Interaction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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