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Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Syk (Tyr525, 526). Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Syk (Tyr525, 526). Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Synonyms | Tyrosine-protein kinase SYK(Spleen tyrosine kinase) [Contains: 72kDa tyrosine-protein kinase SYK, Tyrosine-protein kinase SYK, Tyrosine protein kinase SYK, Syk (p-Y525, 526), Syk (p-Tyr525, 526), Syk, Spleen tyrosine kinase, p-Syk (Y525, 526), p-Syk (Tyr525, 526), p72syk, KSYK, 40 kDa tyrosine-protein kinase SYK |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Rabbit,GuineaPig) |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syk (Tyr525 + Tyr526)) Polyclonal Antibody, Unconjugated (TMAB-01514) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane1: Embryo (Mouse) Lysate at 30 μg Lane2: Thymus (Mouse) Cell Lysate at 30 μg Primary: Anti-phospho-Syk (Tyr525/526) (TMAB-01514) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 70 kDa |
Application | |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane. Cytoplasm, cytosol. |
Tissue Specificity | Widely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro-B-cells to plasma cells. |
Construction | Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Concentration | 1 mg/mL |
Research Background | This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
Immunogen | KLH conjugated Synthesised phosphopeptide: human Syk around the phosphorylation site of Tyr525/526 |
Antigen Species | Human |
Gene Name | SYK |
Gene ID | |
Protein Name | Tyrosine-protein kinase SYK |
Uniprot ID | |
Biology Area | Platelets,Mast Cells,Tyrosine Kinases |
Function | Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen. |
Molecular Weight | Theoretical: 72 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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