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Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Syk (Tyr525, 526). Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Syk (Tyr525, 526). Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | Tyrosine-protein kinase SYK(Spleen tyrosine kinase) [Contains: 72kDa tyrosine-protein kinase SYK, Tyrosine-protein kinase SYK, Tyrosine protein kinase SYK, Syk (p-Y525, 526), Syk (p-Tyr525, 526), Syk, Spleen tyrosine kinase, p-Syk (Y525, 526), p-Syk (Tyr525, 526), p72syk, KSYK, 40 kDa tyrosine-protein kinase SYK |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Rabbit,GuineaPig) |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syk (Tyr525 + Tyr526)) Polyclonal Antibody, Unconjugated (TMAB-01514) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane1: Embryo (Mouse) Lysate at 30 μg Lane2: Thymus (Mouse) Cell Lysate at 30 μg Primary: Anti-phospho-Syk (Tyr525/526) (TMAB-01514) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 70 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane. Cytoplasm, cytosol. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
Immunogen | KLH conjugated Synthesised phosphopeptide: human Syk around the phosphorylation site of Tyr525/526 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Platelets,Mast Cells,Tyrosine Kinases |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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