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Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571)
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Catalog No. TMAC-03227
Alias p-Hsp27/HSPB (Ser78), p-Hsp27/HSPB (S78), Hsp27/HSPB (p-Ser78), Hsp27/HSPB (p-S78)
Anti-Phospho-Hsp27/HSPB (S78) Antibody (3C571) is a Rabbit antibody targeting Phospho-Hsp27/HSPB (S78). Anti-Phospho-Hsp27/HSPB (S78) Antibody (3C571) can be used in WB,ICC/IF,IHC,IP.
Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571)
Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571)
😃Good
Catalog No. TMAC-03227Alias p-Hsp27/HSPB (Ser78), p-Hsp27/HSPB (S78), Hsp27/HSPB (p-Ser78), Hsp27/HSPB (p-S78)
Anti-Phospho-Hsp27/HSPB (S78) Antibody (3C571) is a Rabbit antibody targeting Phospho-Hsp27/HSPB (S78). Anti-Phospho-Hsp27/HSPB (S78) Antibody (3C571) can be used in WB,ICC/IF,IHC,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571) is a Rabbit antibody targeting Phospho-Hsp27/HSPB (Ser78). Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571) can be used in WB,ICC/IF,IHC,IP. |
| Synonyms | p-Hsp27/HSPB (Ser78), p-Hsp27/HSPB (S78), Hsp27/HSPB (p-Ser78), Hsp27/HSPB (p-S78) |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Phospho-Hsp27 (S78) on different lysates using anti-Phospho-Hsp27 (S78) antibody at 1/1,000 dilution. Positive control: Lane 1: Human heart, Lane 2: Human skeletal muscle. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Hsp27 (S78) antibody. Counter stained with hematoxylin. 3. ICC staining Phospho-Hsp27 (S78) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Phospho-Hsp27 (S78) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Phospho-Hsp27 (S78) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. Heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. The intracellular concentration of the mammalian heat shock protein, HSP 27, increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A synthesized phosphopeptide: human Hsp27 around the phosphorylation site of Ser78 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 27 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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